I suspect that if any global feedback was disconnected, then measurement of output distortion as signal rises would be a good determinant of output stage valve matching, as it measures a consequence of imbalance across the whole dynamic range - both from the valves and their imbalance effect on the OT.
It would be interesting to know if anyone has checked this for a reasonable number of matched/unmatched pairs to appreciate the spread in distortion levels experienced.
Another measurement for fixed bias PP could be to meassure the voltage signal across individual cathode sense resistors, and do a precision rectification of each signal, and compare the DC levels through each PP valve. For large signal levels, this may be a good indicator of the DC biasing actually being experienced by the OT (compared with just matching at idle level).
A current transducer that can meassure DC at low levels may also be able to measure such imbalance - eg. by passing both anode leads antiphase through the transducer (eg. a LEM, or tek current probe).
I guess another way could be to apply a dc current to an auxiliary winding on the OT (eg. use a separate secondary if available, or wind a separate turn or two if accessible), and then check if output distortion is minimised with bias.